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Expression data of Arabidopsis wild type Col and mutant pcfs4-1

ABSTRACT: Arabidopsis polyadenylation factor PCFS4 functioned in the alternative polyadenylation of FCA. To identify additional genes differetially processed by PCFS4, we compared the transcriptomes of PCFS4 mutant, pcfs4-1 and wild type Col with Arabidopsis tiling array analyses and a newly developed data processing protocol, RADPRE. We found 68 genes were differentially processed and 114 genes differetially expressed in pcfs4-1 mutant. The top 7 of 68 differetially processed genes were further confirmed with real-time RT-PCR analysis. Arabidopsis plants were grown in a radomized block design with 3 replicates for each of pcfs4-1 and Col. Each block contained one pcfs4-1 and one Col. above ground tissue of 15-day old seedlings was collected 8 hours after dawn. Total RNAs were extracted using Concert Plant RNA reagent (Invitrogen); The double-stranded cDNA targets prepared from total RNA were labeled and hybridized to the Arabidopsis Tiling 1.0R array.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Quinn Li

PROVIDER: E-GEOD-21250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data of Arabidopsis wild type Col and mutant pcfs4-1

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