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RNA-Seq of Drosophila cell line Dmel2

ABSTRACT: Deep Sequencing of mRNA from the Drosophila melanogaster cell line D.Mel-2 Analysis of poly(A)+ RNA of D.Mel-2 cell line

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michael Boutros

PROVIDER: E-GEOD-21283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Design and evaluation of genome-wide libraries for RNA interference screens.

Horn Thomas T Sandmann Thomas T Boutros Michael M

Genome biology 20100615 6

RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/. ...[more]

PMID: 20550664

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