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MiRNA signatures of intracellular and extracellular fractions from cancer cell lines

ABSTRACT: For the purpose of finding a novel tumor marker, we conducted microRNA profiling analysis in fractions of cells, exosomes, and culture medium from seven cancer cell lines. Ranking data of the intracellular and extracellular microRNAs obtained by microarray analysis, we found that let-7 microRNA family are rich in all fractions from AZ-P7a cells, a metastatic gastric cancer cell line. miRNA profiling in fractions of cells, exosomes, and culture medium were conducted by miRNA microarray analysis. Two replicates of intra-samples were performed.

ORGANISM(S): Homo sapiens

SUBMITTER: Keiichi HATAKEYAMA

PROVIDER: E-GEOD-21350 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line.

Ohshima Keiichi K Inoue Kanako K Fujiwara Akemi A Hatakeyama Keiichi K Kanto Kaori K Watanabe Yuko Y Muramatsu Koji K Fukuda Yorikane Y Ogura Shun-ichiro S Yamaguchi Ken K Mochizuki Tohru T

PloS one 20101008 10

<h4>Background</h4>Exosomes play a major role in cell-to-cell communication, targeting cells to transfer exosomal molecules including proteins, mRNAs, and microRNAs (miRNAs) by an endocytosis-like pathway. miRNAs are small noncoding RNA molecules on average 22 nucleotides in length that regulate numerous biological processes including cancer pathogenesis and mediate gene down-regulation by targeting mRNAs to induce RNA degradation and/or interfering with translation. Recent reports imply that mi ...[more]

PMID: 20949044

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