Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Leishmania RNA Virus controls the severity of muco-cutaneous leishmaniasis


ABSTRACT: Comparison of the transcriptome of bone marrow derived macrophages infected with either metastasizing (M+, Lg13) or non-metastasizing (M-, Lg17) parasites after 24 hours of infection. 3 experimental conditions; M+ infected BMMf vs. uninfected BMMf, M- infected BMMf vs. uninfected BMMf, M+ infected BMMf vs. M- infected BMMf, BMMf from 3 biologically independant infection experiments were used where BMMf were infected for 24hrs at a ratio of 1 BMMf to 10 parasites. Total RNA was extracted reverse transcribed into cDNA with either a Cy3 or Cy5 fluorescent tag and hybridized on to mouse 17k chip. Slides were scanned and differential BMMf gene expression was determined above 1.5 fold change and p less than 0.05. 6 slides per biological independant experiment were used including the dye swap, with 18 slides used in total.

ORGANISM(S): Mus musculus

SUBMITTER: Frédéric Schütz 

PROVIDER: E-GEOD-21418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2011-01-03 | GSE21418 | GEO
2011-12-01 | E-GEOD-24791 | biostudies-arrayexpress
2016-02-27 | E-GEOD-78700 | biostudies-arrayexpress
2008-10-26 | E-GEOD-12963 | biostudies-arrayexpress
2021-05-15 | MSV000087437 | MassIVE
| PRJNA102883 | ENA
2007-11-01 | E-MEXP-1290 | biostudies-arrayexpress
| PRJNA101891 | ENA
2016-03-15 | E-GEOD-78888 | biostudies-arrayexpress
2021-09-09 | PXD021925 | Pride