Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Markus Hafner
PROVIDER: E-GEOD-21577 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Cell 20100401 1
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thio ...[more]