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Gene expression study of macrophages during early foreign body reaction

ABSTRACT: Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of macrophages in the development of FBR. This study profiles gene expression of in vitro fibrinogen activated macrophages as well as that of freshly isolated macrophages from 3-days implants, against a background of unactivated macrophages/monocytes. Total RNA was isolated from fibrinogen activated macrophages, FBR macrophages and macrophages from blood (control). Samples were processed in triplicate on Rat Genome 230 2.0 Array GeneChips, adding up to 9 arrays in total. Microarray processing was performed at DNAVision (Charleroi, Belgium). The RMA comparisons between macrophases are linked below.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Lindsay Mesure

PROVIDER: E-GEOD-21682 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts.

Mesure Lindsay L De Visscher Geofrey G Vranken Ilse I Lebacq An A Flameng Willem W

PloS one 20100923 9

Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantat ...[more]

PMID: 20886081

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2013-01-23 | GSE21682 | GEO

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