Genome-wide identification of Streptococcus pneumoniae genes essential for the development of experimental meningitis

ABSTRACT: We applied genomic array footprinting (GAF) in the search for genes of S. pneumoniae essential during the establishment and progression of experimental meningitis. Four libraries with a total of 6,000 independent TIGR4 marinerT7 transposon mutants were injected intra-cisternally in rabbits, and cerebrospinal fluid (CSF) was collected after three, nine, and fifteen hours. Microarray analysis of mutant-specific probes from CSF samples and inocula identified 82 genes of which mutants had become attenuated and eleven genes of which mutants had become enriched during infection. Screening results particularly point to an essential role for capsular polysaccharides (cps), nutrient uptake, and metabolism in meningitis. Detailed study on directed mutants of a subset of sixteen GAF targets in an exp

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Peter Burghout 

PROVIDER: E-GEOD-21729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress