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Transcription profiling of human monocytes from pre-and post-menopausal women with high or low bone mineral density

ABSTRACT: Monocytes were isolated from 30 ml of whole blood from each of 19 women, 10 with high BMD and 9 with low BMD, using monocyte negative isolation kit from Dynal Biotech Inc. Total RNA was extracted from monocytes using Qiagen RNeasy Mini Kit. Targets were produced for each subject using standard Affymetrix procedures from about 4ug of total RNA. Hybridization was made for each subject. Comparison was performed between 10 high BMD and 9 low BMD subjects.

ORGANISM(S): Homo sapiens

DISEASE(S): high bone mineral density

SUBMITTER: Yaozhong Liu

PROVIDER: E-GEOD-2208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A novel pathophysiological mechanism for osteoporosis suggested by an in vivo gene expression study of circulating monocytes.

Liu Yao-Zhong YZ Dvornyk Volodymyr V Lu Yan Y Shen Hui H Lappe Joan M JM Recker Robert R RR Deng Hong-Wen HW

The Journal of biological chemistry 20050617 32

Bone mineral density (BMD) is a major risk factor for osteoporosis. Circulating monocytes may serve as early progenitors of osteoclasts and produce a wide variety of factors important to bone metabolism. However, little is known about the roles of circulating monocytes in relation to the pathophysiology of osteoporosis. Using the Affymetrix HG-U133A GeneChip(R) array, we performed a comparative gene expression study of circulating monocytes in subjects with high and low BMD. We identified in tot ...[more]

PMID: 15965235

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gene expression study of blood monocytes

Project description:Monocytes were isolated from 30 ml of whole blood from each of 19 women, 10 with high BMD and 9 with low BMD, using monocyte negative isolation kit from Dynal Biotech Inc. Total RNA was extracted from monocytes using Qiagen RNeasy Mini Kit. Targets were produced for each subject using standard Affymetrix procedures from about 4ug of total RNA. Hybridization was made for each subject. Comparison was performed between 10 high BMD and 9 low BMD subjects. Keywords = microarray Keywords = monocyte Keywords = osteoporosis Keywords = HDC Keywords = CCR3 Keywords = GCR Keywords: other

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Transcription profiling of human circulating B Lymphocytes from patients with high or low bone density

Project description:B cells produce important cytokines regulate bone metabolism. We comparison gene expression patterns of circulating B cells in blood from 20 postmenopausal females with low or high bone mineral density (BMD): 10 low BMD vs. 10 high BMD. In total 29 differentially expressed genes were identified including some novel genes to be relevant to bone metabolism. These results provide insight into the role of B cells in pathologic osteoporosis. Experiment Overall Design: B cells were isolated from 70 ml of whole blood from each of 20 women, 10 with high BMD and 10 with low BMD, using B cell positive isolation method (Dynabeads CD19,Pan B) from Invitrogen Life Technologies Dynal Biotech Inc,CA. Total RNA was extracted from B cells using Qiagen RNeasy Mini Kit.A total of 4ug totalRNA was used to produced targets for each subject according to standard Affymetrix procedures. Hybridization was made for each subject. Robust Multiarray Algorithm was used to normalize the expression data and comparison was performed between high BMD and low BMD subgroups using t-statistics under mutiple-testing adjustment.

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