Transcription profiling of neutrophils isolated by microfluidics: Clinical Microfluidics for Neutrophil Genomics and Proteomics

ABSTRACT: Neutrophils play critical roles in modulating the immune response. However, neutrophils have a short circulating half life, are readily stimulated in vitro, and have low levels of cellular mRNA when compared to other blood leukocyte populations. All of these factors have made it difficult to evaluate neutrophils from clinical populations for molecular and functional studies. Here we present a robust methodology for rapidly isolating neutrophils directly from whole blood and develop ‘on- chip’ processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and demonstrate the ability of the device to discriminate subtle differences in the genomic and proteomic response of peripheral blood neutrophils to direct and in

ORGANISM(S): Homo sapiens

SUBMITTER: Wenzhong Xiao 

PROVIDER: E-GEOD-22103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress