Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Calcium is a global regulator in Vibrio parahaemolyticus

ABSTRACT: Here we probe the global response to calcium in the marine bacterium and pathogen Vibrio parahaemolyticus by using transcriptome, reporter and phenotypic analyses. Swarming gene expression and motility were enhanced by calcium. Calcium also stimulated expression and function of one of the organismM-bM-^@M-^Ys two type three secretion systems (T3SS1 but not the T3SS2). Although low calcium is an inducing signal for the T3SS of many organisms, calcium stimulation of T3SS has not been reported before. EGTA was also a stimulus for T3SS1 expression and activity; however this appeared to be the consequence of iron rather than calcium chelation. LafK, a key regulator of swarming genes, was found necessary for calcium induction of T3SS1 gene transcription and cytotoxicity. Regulation of swarming and T3SS1 were additionally linked by a negative feedback loop propagated by ExsA. Overexpression of exsA was used to probe the extent of the T3SS1 regulon and verify its coincident induction by calcium and EGTA. The calcium transcriptome analysis revealed a calcium-repressed LysR-type transcriptional regulator; CalR was shown to repress swarming and T3SS1 gene expression. Thus in V. parahaemolyticus, calcium influences gene expression and behavior and seems a signal pertinent for surface colonization and virulence. The gene expression profiles of Vibrio parahaemolyticus cells grown on rich medium or medium supplemented with CaCl2 or EGTA were compared using Affymetrix custom microarrays.

ORGANISM(S): Vibrio parahaemolyticus

SUBMITTER: Linda McCarter

PROVIDER: E-GEOD-22190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Calcium and iron regulate swarming and type III secretion in Vibrio parahaemolyticus.

Gode-Potratz Cindy J CJ Chodur Daniel M DM McCarter Linda L LL

Journal of bacteriology 20100917 22

Here, we probe the response to calcium during growth on a surface and show that calcium influences the transcriptome and stimulates motility and virulence of Vibrio parahaemolyticus. Swarming (but not swimming) gene expression and motility were enhanced by calcium. Calcium also elevated transcription of one of the organism's two type III secretion systems (T3SS1 but not T3SS2) and heightened cytotoxicity toward host cells in coculture. Calcium stimulation of T3SS gene expression has not been rep ...[more]

PMID: 20851895

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Dataset Information

Calcium is a global regulator in Vibrio parahaemolyticus

Publications

Calcium and iron regulate swarming and type III secretion in Vibrio parahaemolyticus.

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