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Expression data of Soybean (Glycine max) roots from different iron treatments.

ABSTRACT: Two Near Isogenic soybean (Glycine max) lines were grown in hydroponic conditions with either 50uM ferric nitrate or 100uM ferric nitrate. After 10 days, half the plants were harvested (total root tissue). At 12 days after planting, iron was added to plants grown in low iron conditions bringing them up to sufficient iron growth conditions. Root tissue was harvested for the remaining plants at 14 days after planting. Gene expression analysis from root tissue of two Near Isogenic Lines (NILs), Clark (PI548553) and IsoClark (PI547430), grown in iron stress or iron stress recovered conditions. A total of 24 samples from four growth conditions, three biological replicates per treatment

ORGANISM(S): Glycine max

SUBMITTER: Carroll Vance

PROVIDER: E-GEOD-22227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An integrative approach to genomic introgression mapping.

Severin Andrew J AJ Peiffer Gregory A GA Xu Wayne W WW Hyten David L DL Bucciarelli Bruna B O'Rourke Jamie A JA Bolon Yung-Tsi YT Grant David D Farmer Andrew D AD May Gregory D GD Vance Carroll P CP Shoemaker Randy C RC Stupar Robert M RM

Plant physiology 20100723 1

Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generat ...[more]

PMID: 20656899

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