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Effect of TIF1γ gene deletion on hematopoietic stem cells

ABSTRACT: Analysis of KLS cells purified from bone marrow of mice conditionally inactivated for TIF1γ gene. TIF1γ deletion results in multiple defects in adult hematopoiesis. Results provide insight into the role of TIF1γ in hematopoietic stem cells functions. We used microarrays to characterize the molecular mechanisms underlying the biological properties of KLS cells lacking TIF1γ KLS (lin-,c-kit+, Sca+) cells were purified by cell sorting

ORGANISM(S): Mus musculus

SUBMITTER: Doulaye Dembele

PROVIDER: E-GEOD-22280 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adult hematopoiesis is regulated by TIF1γ, a repressor of TAL1 and PU.1 transcriptional activity.

Kusy Sophie S Gault Nathalie N Ferri Federica F Lewandowski Daniel D Barroca Vilma V Jaracz-Ros Agnieszka A Losson Regine R Romeo Paul-Henri PH

Cell stem cell 20110401 4

Crosstalk between transcription factors and cytokines precisely regulates tissue homeostasis. Transcriptional intermediary factor 1γ (TIF1γ) regulates vertebrate hematopoietic development, can control transcription elongation, and is a component of the TGF-β signaling pathway. Here we show that deletion of TIF1γ in adult hematopoiesis is compatible with life and long-term maintenance of essential blood cell lineages. However, loss of TIF1γ results in deficient long-term hematopoietic stem cell ( ...[more]

PMID: 21474105

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