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Expression data from CD11cHI and CD11cLO decidual macrophage populations.

ABSTRACT: Decidual macrophage populations, CD11cHI and CD11cLO cells were analyzed for expression profiles and unique characteristics. We used microarrays to detail the global program of gene expression and to determine differences between these two unique decidual macrophage populations. First trimester decidual tissue was digested in order to release immune cells. Cells were stained for CD14 and CD11c and purified by MoFlo sorting. Cells were immediately lysed for RNA extraction and hybridization on Affymetrix microarrays. We utilized 8 patient paired samples (total of 16 microarrays).

ORGANISM(S): Homo sapiens

SUBMITTER: Brandy Houser

PROVIDER: E-GEOD-22342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Two unique human decidual macrophage populations.

Houser Brandy L BL Tilburgs Tamara T Hill Jonathan J Nicotra Matthew L ML Strominger Jack L JL

Journal of immunology (Baltimore, Md. : 1950) 20110121 4

Several important events occur at the maternal-fetal interface, including generation of maternal-fetal tolerance, remodeling of the uterine smooth muscle and its spiral arteries and glands, and placental construction. Fetal-derived extravillous trophoblasts come in direct contact with maternal decidual leukocytes. Macrophages represent ∼20% of the leukocytes at this interface. In this study, two distinct subsets of CD14(+) decidual macrophages (dMs) are found to be present in first-trimester dec ...[more]

PMID: 21257965

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Expression data from CD11cHI and CD11cLO decidual macrophage populations.

Project description:Decidual macrophage populations, CD11cHI and CD11cLO cells were analyzed for expression profiles and unique characteristics. We used microarrays to detail the global program of gene expression and to determine differences between these two unique decidual macrophage populations.

2011-05-23 | GSE22342 | GEO

Homo sapiens

Project description:Expression data from CD11cHI and CD11cLO decidual macrophage populations.

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Development of decidual cell polyploidy and bi-nucleation necessitates mitochondrial activity

Project description:In the present study, to better define the aspects of decidual cell polyploidy, we isolated pure polyploid and non-polyploid decidual cell populations from the in vivo decidual bed. Three independent RNA pools prepared for each population were then subjected to the Affymetrix gene chip analysis for the whole mouse genome transcripts. Our data revealed up-regulation of 1015 genes and down-regulation of 1207 genes in the polyploid populations, as compared to the non-polyploid group.

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Development of decidual cell polyploidy and bi-nucleation necessitates mitochondrial activity

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Project description:Decidual glycodelin-A polarizes human monocytes into a decidual macrophage-like phenotype through Siglec-7

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Gene expression profiling of human decidual macrophages

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