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Selective control of metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae

ABSTRACT: When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. We examine how yeast growth and tRNA charging or aminoacylation is affected during amino acid depletion in the presence and absence of GCN2. tRNA charging is measured using a microarray technique which allows for simultaneous measurement of all cytosolic tRNAs. A fully prototrophic and its isogenic GCN2 deletion strain were used. We measured relative tRNA charging levels in yeast strains with an intact and deleted GCN2.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Zaborske

PROVIDER: E-GEOD-22452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Selective control of amino acid metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae.

Zaborske John M JM Wu Xiaochen X Wek Ronald C RC Pan Tao T

BMC biochemistry 20100804

<h4>Background</h4>When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. Activated Gcn2p up-regulates the general amino acid control pathway through phosphorylation of the translational initiation factor eIF2. In Saccharomyces cerevisiae, Gcn2p is the only kinase that phosphorylates eIF2 to regulate translation through this mechanism. We addressed changes in yeast growth and tRNA aminoacylation, or charging, during amino aci ...[more]

PMID: 20684782

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