Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Arabidopsis Whole Genome 36-mer


ABSTRACT: Identification of novel transcribed sequences in Arabidopsis thaliana using high density genome tiling arrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Waraporn Tongprasit 

PROVIDER: E-GEOD-2247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of transcribed sequences in Arabidopsis thaliana by using high-resolution genome tiling arrays.

Stolc Viktor V   Samanta Manoj Pratim MP   Tongprasit Waraporn W   Sethi Himanshu H   Liang Shoudan S   Nelson David C DC   Hegeman Adrian A   Nelson Clark C   Rancour David D   Bednarek Sebastian S   Ulrich Eldon L EL   Zhao Qin Q   Wrobel Russell L RL   Newman Craig S CS   Fox Brian G BG   Phillips George N GN   Markley John L JL   Sussman Michael R MR  

Proceedings of the National Academy of Sciences of the United States of America 20050308 12


Using a maskless photolithography method, we produced DNA oligonucleotide microarrays with probe sequences tiled throughout the genome of the plant Arabidopsis thaliana. RNA expression was determined for the complete nuclear, mitochondrial, and chloroplast genomes by tiling 5 million 36-mer probes. These probes were hybridized to labeled mRNA isolated from liquid grown T87 cells, an undifferentiated Arabidopsis cell culture line. Transcripts were detected from at least 60% of the nearly 26,330 a  ...[more]

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