Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis polysomal and non polysomal RNAs from plants exposed to mild dehydration vs. controls


ABSTRACT: Microarray experiment with polysomal and non-polysomal RNAs extracted under non-stress and mild-dehydration stress.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Julia Bailey-Serres 

PROVIDER: E-GEOD-2268 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

mRNA sequence features that contribute to translational regulation in Arabidopsis.

Kawaguchi Riki R   Bailey-Serres Julia J  

Nucleic acids research 20050216 3


DNA microarrays were used to evaluate the regulation of the proportion of individual mRNA species in polysomal complexes in leaves of Arabidopsis thaliana under control growth conditions and following a mild dehydration stress (DS). The analysis determined that the percentage of an individual gene transcript in polysomes (ribosome loading) ranged from over 95 to <5%. DS caused a decrease in ribosome loading from 82 to 72%, with maintained polysome association for over 60% of the mRNAs with an in  ...[more]

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