Unknown,Transcriptomics,Genomics,Proteomics

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Small RNA profiling of RNase III enzyme deficient DN3 thymocytes, Tregs, activated CD4+ T cells, and embryonic fibroblasts


ABSTRACT: Here we analyze the small RNA species in the following: 1. DN3 thymocytes following inactivation of LoxP flanked Drosha and Dicer alleles with Lck-cre 2. Tregs following inactivation of LoxP flanked Drosha and Dicer alleles with CD4-cre 3. activated CD4+ T cells following inactivation of LoxP flanked Drosha and Dicer alleles with CD4-cre 4. MEFs following inactivation of LoxP flanked Drosha and Dicer alleles with Rosa26-CreER and 4-OH tamoxifen treatment Fractionation of small RNAs (19-30nt) and cloning of only RNA with 5' phosphates and 3' hydroxyls

ORGANISM(S): Mus musculus

SUBMITTER: Mark Chong 

PROVIDER: E-GEOD-22760 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Canonical and alternate functions of the microRNA biogenesis machinery.

Chong Mark M W MM   Zhang Guoan G   Cheloufi Sihem S   Neubert Thomas A TA   Hannon Gregory J GJ   Littman Dan R DR  

Genes & development 20100816 17


The canonical microRNA (miRNA) biogenesis pathway requires two RNaseIII enzymes: Drosha and Dicer. To understand their functions in mammals in vivo, we engineered mice with germline or tissue-specific inactivation of the genes encoding these two proteins. Changes in proteomic and transcriptional profiles that were shared in Dicer- and Drosha-deficient mice confirmed the requirement for both enzymes in canonical miRNA biogenesis. However, deficiency in Drosha or Dicer did not always result in ide  ...[more]

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