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NCI60 Expression Profiling using the Agilent Whole Human Genome Oligo Microarray

ABSTRACT: We present here a new expression profiling study of the 60 cell lines of the National Cancer Institute (NCI) Developmental Therapeutics program (DTP) drug screen (NCI-60) using the 41,000-probe Agilent Whole Human Genome Oligo Microarray. The expression levels of ∼21,000 genes were measured. The profiling study includes quadruplicate technical replicates for six cell lines and duplicates for the remaining cell lines. The resulting data sets are freely available and searchable online in the CellMiner database (http://discover.nci.nih.gov).

ORGANISM(S): Homo sapiens

SUBMITTER: Hongfang Liu

PROVIDER: E-GEOD-22821 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Coordinated regulation of mitochondrial topoisomerase IB with mitochondrial nuclear encoded genes and MYC.

Zoppoli Gabriele G Douarre Céline C Dalla Rosa Ilaria I Liu Hongfang H Reinhold William W Pommier Yves Y

Nucleic acids research 20110429 15

Mitochondrial DNA (mtDNA) is entirely dependent on nuclear genes for its transcription and replication. One of these genes is TOP1MT, which encodes the mitochondrial DNA topoisomerase IB, involved in mtDNA relaxation. To elucidate TOP1MT regulation, we performed genome-wide profiling across the 60-cell line panel (the NCI-60) of the National Cancer Institute Developmental Therapeutics Program. We show that TOP1MT mRNA expression varies widely across these cell lines with the highest levels in le ...[more]

PMID: 21531700

Publication: 1/2

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