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Global expression profiling of peripheral Qa-1 class Ib-restricted CD8aa+TCRab+ regulatory T Cells reveals innate-like features: implications for immune regulatory repertoire

ABSTRACT: To better understand the function of CD8aa Tregs, we have recently characterized several CD8aa+TCRab+ T cell clones and lines that are physiologically primed and are involved in recovery and protection from EAE (REF). In this report, we present a comparison of global gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells. The results of microarray data analysis are confirmed by real-time PCR and flow cytometry for better accuracy and phenotype expression. Our study reveals a unique gene signature in the CD8aa Tregs, including enhanced expression of several inhibitory molecules associated with the maintenance of peripheral T cell homeostasis. The identification of differentially expressed molecules by CD8aa Tregs should facilitate future functional studies in this area. gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells

ORGANISM(S): Mus musculus

SUBMITTER: Radu Cojocaru 

PROVIDER: E-GEOD-22985 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global expression profiling of peripheral Qa-1-restricted CD8αα+TCRαβ+ regulatory T cells reveals innate-like features: implications for immune-regulatory repertoire.

Fanchiang Shaohsuan S SS   Cojocaru Radu R   Othman Mohammad M   Khanna Ritu R   Brooks Matthew J MJ   Smith Trevor T   Tang Xiaolei X   Maricic Igor I   Swaroop Anand A   Kumar Vipin V  

Human immunology 20110817 3

Among peripheral regulatory T cells, CD8(+) T cells also play an important role in the maintenance of immune homeostasis. A subset of CD8(+) Treg that express αβ T cell receptor (TCR) and CD8αα homodimers can recognize TCR-derived peptides in the context of the class Ib MHC molecule Qa-1. To gain a better understanding of the nature and phenotype of CD8αα(+)TCRαβ+ Treg, a global gene expression profiling using microarray, real-time quantitative polymerase chain reaction, and flow-cytometric anal  ...[more]

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