Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Global expression profiling of peripheral Qa-1 class Ib-restricted CD8aa+TCRab+ regulatory T Cells reveals innate-like features: implications for immune regulatory repertoire

ABSTRACT: To better understand the function of CD8aa Tregs, we have recently characterized several CD8aa+TCRab+ T cell clones and lines that are physiologically primed and are involved in recovery and protection from EAE (REF). In this report, we present a comparison of global gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells. The results of microarray data analysis are confirmed by real-time PCR and flow cytometry for better accuracy and phenotype expression. Our study reveals a unique gene signature in the CD8aa Tregs, including enhanced expression of several inhibitory molecules associated with the maintenance of peripheral T cell homeostasis. The identification of differentially expressed molecules by CD8aa Tregs should facilitate future functional studies in this area. gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells

ORGANISM(S): Mus musculus

SUBMITTER: Radu Cojocaru 

PROVIDER: E-GEOD-22985 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Global expression profiling of peripheral Qa-1-restricted CD8αα+TCRαβ+ regulatory T cells reveals innate-like features: implications for immune-regulatory repertoire.

Fanchiang Shaohsuan S SS   Cojocaru Radu R   Othman Mohammad M   Khanna Ritu R   Brooks Matthew J MJ   Smith Trevor T   Tang Xiaolei X   Maricic Igor I   Swaroop Anand A   Kumar Vipin V  

Human immunology 20110817 3

Among peripheral regulatory T cells, CD8(+) T cells also play an important role in the maintenance of immune homeostasis. A subset of CD8(+) Treg that express αβ T cell receptor (TCR) and CD8αα homodimers can recognize TCR-derived peptides in the context of the class Ib MHC molecule Qa-1. To gain a better understanding of the nature and phenotype of CD8αα(+)TCRαβ+ Treg, a global gene expression profiling using microarray, real-time quantitative polymerase chain reaction, and flow-cytometric anal  ...[more]

Similar Datasets

Transcriptomics Global expression profiling of peripheral Qa-1 class Ib-restricted CD8aa+TCRab+ regulatory T Cells reveals innate-like features: implications for immune regulatory repertoire
2011-09-01 | GSE22985 | GEO
Transcriptomics Functional Analysis and Gene Expression Profile of Umbilical Cord Blood Regulatory T Cells
2010-12-31 | E-GEOD-22501 | biostudies-arrayexpress
Transcriptomics Distinct signature of altered homeostasis in aging rod photoreceptors: Implications for retinal diseases
2010-06-14 | E-GEOD-22317 | biostudies-arrayexpress
Transcriptomics Single-cell RNA sequencing reveals peripheral blood mononuclear immune cell landscape associated with operational tolerance in a kidney transplant recipient [2]
2023-06-20 | GSE230650 | GEO
Transcriptomics Single-cell RNA sequencing reveals peripheral blood mononuclear immune cell landscape associated with operational tolerance in a kidney transplant recipient [1]
2023-06-20 | GSE217762 | GEO
Transcriptomics Differentially activated CD8 T cells in liver and gut
2014-02-25 | E-GEOD-39449 | biostudies-arrayexpress
Transcriptomics Classical dendritic cells are required for dietary antigen-mediated peripheral regulatory T cell and tolerance induction II
2016-03-28 | E-GEOD-77304 | biostudies-arrayexpress
Transcriptomics Regulatory T cells suppress the formation of super-effector CD8 T cells by limiting IL-2
2022-01-17 | GSE183940 | GEO
Transcriptomics Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes
2022-03-30 | GSE186164 | GEO
Clinical Circulating Regulatory Lymphocytes and Outcome of Metastatic Colorectal Cancer Patients
| 2120409 | ecrin-mdr-crc