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DNA microarrays (time course) in the iPS process treated with microRNA clusters

ABSTRACT: Treatment with MicroRNA cluster B and C increase the iPS efficiency We used microarrays to identify changes induced by MicroRNA clusters in the iPS process One group were MEFs infected with SKO factors, plus MicroRNA cluster B, C or control blank virus. The other group were MEFs infected with MicroRNA cluster B, C or control blank virus only.TRIZOL cell lysates were prepared at D4 and D8.

ORGANISM(S): Mus musculus

SUBMITTER: Duanqing Pei

PROVIDER: E-GEOD-23104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition.

Liao Baojian B Bao Xichen X Liu Longqi L Feng Shipeng S Zovoilis Athanasios A Liu Wenbo W Xue Yanting Y Cai Jie J Guo Xiangpeng X Qin Baoming B Zhang Ruosi R Wu Jiayan J Lai Liangxue L Teng Maikun M Niu Liwen L Zhang Biliang B Esteban Miguel A MA Pei Duanqing D

The Journal of biological chemistry 20110322 19

MicroRNAs (miRNAs) are emerging critical regulators of cell function that frequently reside in clusters throughout the genome. They influence a myriad of cell functions, including the generation of induced pluripotent stem cells, also termed reprogramming. Here, we have successfully delivered entire miRNA clusters into reprogramming fibroblasts using retroviral vectors. This strategy avoids caveats associated with transient transfection of chemically synthesized miRNA mimics. Overexpression of 2 ...[more]

PMID: 21454525

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