Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Dm_y[1]cn[1] bw[1] sp[1]_pupa_WhitePrePupaep2days_TotalRNA_p200_344-346-348_38bp


ABSTRACT: modENCODE_submission_541 This submission comes from a modENCODE project of Susan Celniker. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: Y cn bw sp; Developmental Stage: Pupae, WPP + 2 days; Genotype: y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1]; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain Y cn bw sp; Developmental Stage Pupae, WPP + 2 days

ORGANISM(S): Drosophila melanogaster

SUBMITTER: DCC modENCODE 

PROVIDER: E-GEOD-23223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The developmental transcriptome of Drosophila melanogaster.

Graveley Brenton R BR   Brooks Angela N AN   Carlson Joseph W JW   Duff Michael O MO   Landolin Jane M JM   Yang Li L   Artieri Carlo G CG   van Baren Marijke J MJ   Boley Nathan N   Booth Benjamin W BW   Brown James B JB   Cherbas Lucy L   Davis Carrie A CA   Dobin Alex A   Li Renhua R   Lin Wei W   Malone John H JH   Mattiuzzo Nicolas R NR   Miller David D   Sturgill David D   Tuch Brian B BB   Zaleski Chris C   Zhang Dayu D   Blanchette Marco M   Dudoit Sandrine S   Eads Brian B   Green Richard E RE   Hammonds Ann A   Jiang Lichun L   Kapranov Phil P   Langton Laura L   Perrimon Norbert N   Sandler Jeremy E JE   Wan Kenneth H KH   Willingham Aarron A   Zhang Yu Y   Zou Yi Y   Andrews Justen J   Bickel Peter J PJ   Brenner Steven E SE   Brent Michael R MR   Cherbas Peter P   Gingeras Thomas R TR   Hoskins Roger A RA   Kaufman Thomas C TC   Oliver Brian B   Celniker Susan E SE  

Nature 20101222 7339


Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental  ...[more]

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