Embryo_GABA_motor_neurons_tiling_array
Ontology highlight
ABSTRACT:
ORGANISM(S): Caenorhabditis elegans
SUBMITTER: DCC modENCODE
PROVIDER: E-GEOD-23258 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:modENCODE_submission_455 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: NW1229 (engineered, target gene F25B3.3 tagged by GFP); Tissue: panneural; Developmental Stage: Mixed stage of embryos 20dC; Genotype: evIs111 [F25B3.3::GFP]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain NW1229 (engineered, target gene F25B3.3 tagged by GFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue panneural
2010-08-04 | E-GEOD-23245 | biostudies-arrayexpress
Project description:modENCODE_submission_654 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: NC300 (engineered, target gene unc-119 tagged by GFP); Tissue: unc-4 neurons (embryonic); Developmental Stage: Mixed stage of embryos 20dC; Genotype: wdIs5[unc-4::GFP; dpy-20(e1282)]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain NC300 (engineered, target gene unc-119 tagged by GFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue unc-4 neurons (embryonic)
2010-08-04 | E-GEOD-23278 | biostudies-arrayexpress
Project description:modENCODE_submission_662 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: DM8001 (engineered, target gene dpy-7 tagged by GFP); Tissue: hypodermis; Developmental Stage: Mixed stage of embryos 20dC; Genotype: raIs/[rol-6(SU1006)+Pdpy-7::GFP]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain DM8001 (engineered, target gene dpy-7 tagged by GFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue hypodermis
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2010-08-04 | E-GEOD-23260 | biostudies-arrayexpress
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2010-08-04 | E-GEOD-23285 | biostudies-arrayexpress
Project description:modENCODE_submission_458 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: TV1112 (engineered, target gene unc-122 tagged by RFP); Tissue: coelomocytes; Developmental Stage: Mixed stage of embryos 20dC; Genotype: wyIs58 (opt-3::GFP::RAB-3; unc-122::RFP); Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain TV1112 (engineered, target gene unc-122 tagged by RFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue coelomocytes
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2010-08-04 | E-GEOD-23257 | biostudies-arrayexpress
Project description:modENCODE_submission_3172 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Tissue: reference (early embryo); Developmental Stage: Mixed stage of embryos 20dC; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain N2; temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue reference (early embryo)
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