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SiRNA profiling of human HUVEC cells after WTAP knockdown

ABSTRACT: The experiment studied the effect of knockdown WTAP in HUVEC. two control samples and two WTAP knockdown samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Horiuchi Keiko

PROVIDER: E-GEOD-2327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA.

Horiuchi Keiko K Umetani Michihisa M Minami Takashi T Okayama Hiroto H Takada Shinji S Yamamoto Masayuki M Aburatani Hiroyuki H Reid Patrick C PC Housman David E DE Hamakubo Takao T Kodama Tatsuhiko T

Proceedings of the National Academy of Sciences of the United States of America 20061106 46

Wilms' tumor 1-associating protein (WTAP) has been reported to be a ubiquitously expressed nuclear protein. Although a relation to splicing factors has been postulated, its actual physiological function still remains to be elucidated. To investigate the role of WTAP, we generated WTAP-knockout mice and performed small interfering RNA (siRNA)-mediated knockdown analyses in primary cultured cells. In DNA microarrays using human umbilical vein endothelial cells, WTAP-targeted siRNA treatment result ...[more]

PMID: 17088532

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