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Pathogen_Pluminscens_25dC_24hr_exposure_post-adulthood_N2_tiling_array

ABSTRACT: modENCODE_submission_486 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Young Adult (pre-gravid) 25dC 46 hrs post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: GrowthCondition P. luminescens (Hb); Developmental Stage Young Adult (pre-gravid) 25dC 46 hrs post-L1; Strain N2; age 24

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: DCC modENCODE

PROVIDER: E-GEOD-23272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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pathogen_Efaecalis_25dC_24hr_exposure_post-adulthood_N2_tiling_array

Project description:modENCODE_submission_487 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Young Adult (pre-gravid) 25dC 46 hrs post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: GrowthCondition E. faecalis (OG1RF); Developmental Stage Young Adult (pre-gravid) 25dC 46 hrs post-L1; Strain N2; age 24

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pathogen_Smarcescens_25dC_24hr_exposure_post-adulthood_N2_tiling_array

Project description:modENCODE_submission_489 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Young Adult (pre-gravid) 25dC 46 hrs post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: GrowthCondition S. marcescens (Db11); Developmental Stage Young Adult (pre-gravid) 25dC 46 hrs post-L1; Strain N2; age 24

2010-08-04 | E-GEOD-23275 | biostudies-arrayexpress

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Project description:modENCODE_submission_488 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commercially available genome tiling arrays. To understand how bacterial pathogens taken up by C. elegans may affect transcription, we grew young adult worms on various pathogenic bacterial strains for either 24 or 48 hours and measured transcription levels. Simultaneously, young adult worms were also grown on non-pathogenic bacteria (OP50) as controls. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Young Adult (pre-gravid) 25dC 46 hrs post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: GrowthCondition S. marcescens (Db11); Developmental Stage Young Adult (pre-gravid) 25dC 46 hrs post-L1; Strain N2; age 48

2010-08-04 | E-GEOD-23274 | biostudies-arrayexpress

pathogen_controlOP50_25dC_24hr_exposure_post-adulthood_N2_tiling_array

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2010-08-04 | E-GEOD-23277 | biostudies-arrayexpress

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2010-08-04 | E-GEOD-23276 | biostudies-arrayexpress

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2010-08-04 | E-GEOD-23268 | biostudies-arrayexpress

early_embryo_20dC_0-4hrs_post-fertilization_N2_tiling_array

Project description:modENCODE_submission_476 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: early embryo; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain N2; Developmental Stage early embryo

2010-08-04 | E-GEOD-23265 | biostudies-arrayexpress

male_mid_L4_25dC_36hrs_post-L1_CB4689_tiling_array

Project description:modENCODE_submission_478 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: dpy28(y1);him-8(e1489); Developmental Stage: Male larva mid-L4 25dC 30 hrs post-L1; Genotype: dpy-28(y1);him-8(e1489); Sex: Male; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain dpy28(y1);him-8(e1489); Developmental Stage Male larva mid-L4 25dC 30 hrs post-L1

2010-08-04 | E-GEOD-23267 | biostudies-arrayexpress

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