Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from seven rat tissues at multiple ages

ABSTRACT: Aging progress is distinctly characterized by systematic and progressive decline of physiological functions with increasing age in virtually all tissues or organs. Addressing the patterns of molecular changes in different tissues and how different tissues interact with each other during aging are an important question in aging. This study sampled seven tissues at four ages in rats to measure genome-scale gene expression, which allows global insight into coordination, specificity and/or commonness among different tissues with aging progress. We sampled seven different tissues or organs of male Sprague Dawley rats including hypothalamus, pituitary, adrenal gland, spleen lymphocytes, liver, kidney, and bone at successive ages of 4, 10, 18, and 24 months. For each age, ten individual rats were obtained and seven tissues from the same individual rat were derived every time, and the 5µg of each extracted RNA sample of the same tissue from ten individual rats was equally mixed, then the total amount of 15 µg RNA from this mixture was hybridized to Affymetrix RAE230A GeneChip.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Qi Wang

PROVIDER: E-GEOD-23328 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Genetic analyses suggest that alterations in gene expression at the molecular and tissue levels can have profound effects on aging for multi-cellular organisms. However, much remains unknown about the normal pattern of genetic changes in different tissues and how these tissues interact during aging. To investigate tissue-specific aging systematically, we measured expression profiles of aging in Drosophila melanogaster in seven tissues representing nervous, muscular, digestive, renal, reproductive, and storage systems. In each tissue, we identified hundreds of age-related genes mostly showing gradual changes of transcript levels with age. Age-relatedgenes showed clear tissue-specific transcriptional patterns; less than 10% of age-related genes in each tissue shared expression patterns with any other tissue; less than 20% of age-related biological processes were shared between tissues. A significant portion of tissue-specific age-related genes are those involved in physiological functions regulated by the corresponding tissue. However, limited overlaps of age-related function groups among tissues particularly those involved in proteasome function suggest some common mechanisms of transcription regulation in aging across tissues. This study defined global, temporal and spatial changes associated withaging at the molecular and tissue levels. Analyses indicated that different tissues might age in different patterns or at different rates. This study addressed comprehensively the relationship of age-related changes among different tissues in one organism, providing a foundation to address tissue-specific regulation in aging. RNA was then amplified by a one-step linear amplification protocol to generate amplified RNA (aRNA). Experiment aRNA refers to amplified RNA from flies of 15, 20, 30, 45 and 60 days old, and reference aRNA refers to amplified RNA from flies of 3 days old, and experiment and reference aRNAs were labeled with fluorescent dye Cy3 and Cy5, respectively. For each tissue, RNA from the corresponding tissue of 3-day old flies was used as the reference RNA and expression profiles at each of the five age-points was measured twice by using independently prepared duplicated samples. Seven types of tissues or organs of the male fly strain w1118 , accessory gland, testis, brain, gut, malpighian tubule, dorsal thoracic muscle and abdominal fat body were hand dissected out of flies at age of 3, 15, 20, 30, 45 and 60 days old. Tissues or organs from four males of the same age were pooled together and used for each RNA sample preparation.

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Expression data from seven rat tissues at multiple ages

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