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Mouse infections with Neospora caninum

ABSTRACT: Transcriptional responses occurring in the spleen in response to N. caninum infection compared to uninfected mice Two conditions: infected and un-infected mice; Biological replicates: three (or four) infected and three uninfected mice. One control and one infected per array

ORGANISM(S): Mus musculus

SUBMITTER: John Ellis

PROVIDER: E-GEOD-23520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mouse infections with Neospora caninum

Project description:Transcriptional responses occurring in the spleen in response to N. caninum infection compared to uninfected mice

2011-01-01 | GSE23520 | GEO

Detection of differential gene expression in locust brain infected with the protozoan parasite Neospora caninum using the drosophila gene chip

Project description:To obtain a broader understanding of the effects of the N. caninum on S. gregaria adults, we examined the parasite impact on host gene expression using Affymetrix DNA microarray and Cross Species Hybridisation (CSH) analysis. Since N. caninum infection induced neurological injuries in locusts, it was important to test whether N. caninum infection had altered gene expression within locust brains. The analysis was designed to gain further understanding of the mechanisms for N. caninum neuropathy, and in particular, of the genes responsible for the capacity of N. caninum to establish brain infection. Using a cross species hybridisation (CSH) approach, RNA samples from brains of mock- and N. caninum-infected locusts were labeled and hybridised to Affymetrix Gene Chip Drosophila Genome 2.0 Arrays. Data were analysed using Partek Genomics Suite Version 6.4. Of the 28,593 transcripts represented on the GeneChip, approximately 18,500 were expressed in the N. caninum-infected locust’s brain. PCA quality control analysis shows that the uninfected and infected samples were biologically noisy but generally well separated .

2015-01-28 | E-MTAB-3132 | biostudies-arrayexpress

Neospora caninum RNAseq

2011-02-08 | E-MTAB-549 | biostudies-arrayexpress

[E-MTAB-549] Neospora caninum RNAseq

Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of N. caninum NCLiv. The aim is to make transcriptional landscape maps at different time points at different life cycle stages of N. caninum and compare it with equivalent datasets from the closely related parasite Toxoplasma gondii. ArrayExpress Release Date: 2011-02-08 Person Roles: submitter Person Last Name: Service Person First Name: Submission Person Mid Initials: Person Email: datahose@sanger.ac.uk Person Phone: Person Address: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom Person Affiliation: Wellcome Trust Sanger Institute Person Roles: Investigator Person Last Name: Reid Person First Name: Adam Person Mid Initials: Person Email: ar11@sanger.ac.uk Person Phone: 01223 834244 Person Address: Wellcome Trust Genome Campus,Hinxton,Cambridge. CB10 1SA UK Person Affiliation: Wellcome Trust Sanger Institute Person Roles: Project Coordinator Person Last Name: Sanders Person First Name: Mandy Person Mid Initials: J Person Email: mjs@sanger.ac.uk Person Phone: 01223 834244 Person Address: Wellcome Trust Genome Campus,Hinxton,Cambridge. CB10 1SA UK Person Affiliation: Wellcome Trust Sanger Institute

2011-07-14 | GSE30613 | GEO

Neospora caninum

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Impact of Neospora caninum infection on the transcriptome of cerebrovascular endothelial cells

Project description:We also used Affymetrix array technology to investigate the global alteration in the gene expression of human brain microvascular endothelial cells (hBMECs) and rat brain microvascular endothelial cells (rBMVECs) in response to N. caninum infection at 24 hours post infection.

2020-08-10 | E-MTAB-9411 | biostudies-arrayexpress

Neospora caninum Liverpool

Project description:Neospora caninum genome sequencing project

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microRNA profiling in the parasite Neospora caninum using a high-throughput approach

Project description:Small RNA libraries from N. caninum tachyzoites were analyzed using a high-throughput RNA sequencing technology combined with systematic bioinformatics analysis.

2017-11-20 | GSE107118 | GEO

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