Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Porcine spleen_Streptococcus suis serotype 2 infected_3 days

ABSTRACT: Streptococcus suis serotype 2 (SS2), a major swine pathogen and an emerging zoonotic agent, has greatly challenged global public health. Systematical information about host immune response to the infection is important for understanding the molecular mechanism of diseases. Here, we investigated the global expression changes in spleen following SS2 infection using the Affymetrix Porcine Genechip. Our findings indicate previously unrecognized gene transcription changes in case of SS infection in vivo. Our data should provide new clues for immune response in mammals and identification of candidate genes related to SS resistance. All animal tissue collection procedures were performed according to protocols approved by The Hubei Province, PR China for Biological Studies Animal Care and Use Committee. Piglets at 35 days old were determined to be S.suis 2-free by antibody-based ELISA before artificial bacterial challenges. Each piglet was intranasally challenged with 2×106 colony-forming units (CFU) per millilitre of SS2 strain 05ZY or ΔHP0197. A total of 4 piglets were challenged with 05ZY, 4 were challenged with ΔHP0197, and 4 pigs were used as controls. These three groups were raised in isolated facilities. Nine microarrays were used in the experiment, corresponding to the RNAs from spleen tissues of piglets with arthritis after SS2 infection and spleen tissues of piglets with no clinical signs and three controls.

ORGANISM(S): Sus scrofa

SUBMITTER: Meilin Jin

PROVIDER: E-GEOD-23596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Porcine spleen_Haemophilus Parasuis infected_7days

Project description:Haemophilus parasuis(HPS) is a prominent swine pathogen that causes GlM-CM-$sser's disease characterized by fibrinous polyserositis, meningitis and arthritis; however, the molecular mechanisms underlying disease pathogenesis remains poorly understood, particularly the host counteraction to HPS invasion by the immune system. Here, we investigated the global expression changes in spleen following HPS infection using the Affymetrix Porcine Genechip.Our findings indicate previously unrecognized gene transcription changes in case of HPS infection in vivo and many presumed cascades in the study are clearly merit further investigation. Our data should provide new clues for immune response in mammals and identification of candidate genes related to HPS resistance. All animal tissue collection procedures were performed according to protocols approved by The Hubei Province, PR China for Biological Studies Animal Care and Use Committee. Piglets at 30 days old were determined to be HPS-free by serum indirect haemagglutination test before artificial bacterial challenges. Each piglet was intratracheally challenged with 5M-CM-^W108 colony-forming units (CFU) per millilitre of HPS strain 0165 (serotype 5) . A total of 5 piglets were challenged, and 5 pigs were used as controls. These two groups were raised in isolated facilities. Six microarrays were used in the experiment, corresponding to the RNAs from spleen tissues of three most severe piglets with fibrinous polyserositis, meningitis and arthritis after HPS infection and three controls.

2009-03-26 | E-GEOD-11787 | biostudies-arrayexpress

Mutation Mapping of Streptococcus suis serotype 2 strains

Project description:Streptococcus suis serotype 2 (SS2), an important zoonotic agent, is notorious for causing contagious porcine diseases and human infection. The two outbreaks in China (in 1998 and in 2005) have caused serious economic losses in the pig industry and posed public health for its new toxin shock symptoms (TSS). However, the molecular mechanism of SS2 pathogenicity is still poorly understood. In order to get insights into pathogenecity of SS2, eighteen SS2 strains of different virulence and sources have been subjected to whole genome comparison by NimbleGen CGS arrays Comparative genomic analysis of 18 SS2 strains with 05ZYH33 as reference

2009-08-29 | E-GEOD-17868 | biostudies-arrayexpress

Mouse pancreas development

Project description:Pancreas study with 5 sub-studies: (i) 14 assays (7 done on Affymetrix MGU74Av2 and 7 on MOE430 2.0) looking at 7 different time points in pancreas development, (ii) 2 assays (done on Affymetrix MGU74Av2) looking at tumorgenic cell lines alphaTC and betaTC, (iii) 8 assays (6 done on Affymetrix MGU74Av2 and 2 done on MOE430 2.0) looking at Ngn3 mutant and wildtype pancreas at 3 different embryonic time points in pancreas development, (iv) 3 assays (done on Affymetrix MGU74Av2) looking at embryonic e12.5, newborn pancreas and adult islets, (v) 3 assays (done on Affymetrix MGU74Av2) looking at e11.5 separated pancreatic epithelium and mesenchyme or the intact e11.5 pancreas.

2014-09-11 | E-MTAB-2924 | biostudies-arrayexpress

Comparison of WAT CD34+ vs LAF CD34+

Project description:A catalytic role has been proposed in neoplastic angiogenesis and cancer progression for bone marrow-derived endothelial progenitor cells (EPCs). However, in preclinical and clinical studies the quantitative role of marrow-derived EPCs in cancer vascularization was found to be extremely variable. Adipose tissue represents an attractive source of autologous adult stem cells due to its abundance and surgical accessibility. CD34+cells from Lipotransfer aspirates (LAs) of patients undergoing breast reconstruction after breast cancer surgery were compared with CD34+ cells from Leucapheresis of normal subjects. Two samples of purified CD34+ cells from Lipotransfer Aspirates (LAs) were compared with two samples of CD34+ cells from Leucapheresis samples. The reference were considered the CD34+ RNA from leucapheresis

2011-08-15 | E-GEOD-31415 | biostudies-arrayexpress

Rgg-dependent transcriptional regulation in Streptococcus pyogenes strains MGAS5005 and CS101

Project description:Rgg-dependent transcriptional regulation in Streptococcus pyogenes strains MGAS5005 and CS101 was analyzed during post-exponential phase of growth Keywords: strain comparion, post-exponential growth, rgg mutant Microarray analysis was performed using RNA samples isolated from wild-type MGAS5005 and CS101 strains as well as their rgg mutant strains during post-exponential phase of growth

2010-05-26 | E-GEOD-7341 | biostudies-arrayexpress

Rgg-dependent transcriptional regulation in SF370 Streptococcus pyogenes strain

Project description:Rgg-dependent transcriptional regulation in SF370 Streptococcus pyogenes strain was analyzed during post-exponential phase of growth Keywords: rgg mutant Microarray analysis was performed using RNA samples isolated from both wild-type SF370 and SF370 rgg mutant strains during post-exponential phase of growth

2010-05-26 | E-GEOD-7335 | biostudies-arrayexpress

Expression profiles of novel pancreatic cell lines

Project description:We studied the expression profiles of two different cell lines that were generated in our laboratory from tumors found in mice expressing the SV40 T antigen under the control of the upstream glucokinase promoter. The first cell line, called GKP2 cells, was derived from an insulinoma and expresses insulin and pdx1 along with other markers of beta cells. The second cell line, named GKP4 cells, was derived from periductal tumors that did not express insulin or pdx1 but expressed genes of the endocrine lineage, such as Isl1 and Nkx2.2. Interstingly, a small fraction of the GKP4 cells can spontaneously turn on the expression of insulin and pdx1, indicating that they could be islet cell progenitors. The aim of our project was to identify the genes expressed specifically in GKP4 cells. To do so, we compared the gene expression profiles of GKP4 cells with GKP2 cells using DNA microarray.

2014-07-22 | E-MTAB-2773 | biostudies-arrayexpress

Screening of Streptococcus Suis serotype 2 resistance genes with GWAS and transcriptomic microarray analysis (mRNA)

Project description:Swine streptococcosis has caused great economic loss in the swine industry, and the major pathogen responsible for this disease is Streptococcus Suis serotype 2 (SS2). Disease resistance breeding is a fundamental way of resolving this problem. With the development of GWAS and transcriptomic microarray technology, we now have powerful research tools to identify SS2 resistance genes. In this research, we generated an F2 generation of SS2 resistant C57BL/6 and SS2 susceptive A/J mice. With the F2 generation of these two mice strains and GWAS analysis, we identified 286 significant mouse genome SNPs sites associated with the SS2 resistance trait. Gene expression profiles for C57BL/6 and A/J were analyzed under SS2 infection pressure by microarray. In total, 251 differentially expressed genes were identified between these two mouse strains during SS2 infection. After combining the GWAS and gene expression profile data, we located two genes that were significantly associated with SS2 resistance, which were the UBA domain containing 1 gene (Ubac1) and Epsin 1 gene (Epn 1). GO classification and over-representation analysis revealed nine up-regulated related to immune function, which could potentially be involved in the C57BL/6 SS2 resistance trait. This is the first study to use both SNP chip and gene express profile chip for SS2 resistance gene identification in mouse, and these results will contribute to Swine SS2 resistance breeding.

2018-11-27 | GSE122965 | GEO

Screening of Streptococcus Suis serotype 2 resistance genes with GWAS and transcriptomic microarray analysis (SNP)

2018-11-27 | GSE122963 | GEO

Exosomes mediate protective messages during oxidative stress; role of exosomal shuttle RNA

Project description:Exosomes are small extracellular nano-vesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling of functional RNA from one cell to another. In this study, we show that exosomes, produced by mouse mast cells exposed to oxidative stress, change their mRNA content and also that these exosomes can influence the response of other cells to oxidative stress by providing recipient cells with a resistance against oxidative stress. Finally, we also show that UV-light affect the biological functions associated with exosomes released under oxidative stress. These results argue that exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stimulus. We used microarrays to detail to examine the gene expression underlying the effect of H2O2 on the exosomal RNA and how exosomes isolated from oxidative stress influence the growth of recipient cells during oxidative stress. The mRNA content of the exosomes from normal conditions (MC9 Exo_norm) were compared to that from exosomes from H2O2 conditions (MC9 Exo_H2O2). The mRNA content of recipient cells MC9 were identified before (MC9 cells_norm) and after (MC9 cells_H2O2) addition of exosomes that were isolated from normal and H2O2 conditions.

2011-01-07 | E-GEOD-24886 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data