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The effect of aneuploidy on gene expression

ABSTRACT: Analysis of the gene expression changes observed in aneuploid b1 integrin YYFF MEFs generated as a result of repeated cytokinesis failure compared to tetraploid wild-type MEFs or tetraploid b1YYFF integrin MEFs. The results reveal aneuploidy-specific gene expression changes, both up-and downregulated genes uniquely detected in the aneuploid MEFs. Total RNA isolated from b1 integrin YYFF and B1 wildtype mouse embryonic fibroblasts that had been passaged on laminin 0, 4 or 8 times (L0, L4 or L8)

ORGANISM(S): Mus musculus

SUBMITTER: Gunilla Högnäs

PROVIDER: E-GEOD-23729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Aneuploidy facilitates oncogenic transformation via specific genetic alterations, including Twist2 upregulation.

Högnäs Gunilla G Hämälistö Saara S Rilla Kirsi K Laine Jukka O JO Vilkki Vesa V Murumägi Astrid A Edgren Henrik H Kallioniemi Olli O Ivaska Johanna J

Carcinogenesis 20130520 9

Aneuploidy, deviation from the normal chromosome number, and other chromosomal aberrations are commonly observed in cancer. Integrin-mediated adhesion and dynamic turnover of adhesion sites are required for successful cytokinesis of normal adherent cells and impaired cell division can lead to the generation of cells with abnormal chromosome contents. We find that repeated cytokinesis failure, due to impaired integrin traffic alone, is sufficient to induce chromosome aberrations resulting in the ...[more]

PMID: 23689353

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The effect of aneuploidy on gene expression

Project description:Analysis of the gene expression changes observed in aneuploid b1 integrin YYFF MEFs generated as a result of repeated cytokinesis failure compared to tetraploid wild-type MEFs or tetraploid b1YYFF integrin MEFs. The results reveal aneuploidy-specific gene expression changes, both up-and downregulated genes uniquely detected in the aneuploid MEFs.

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b1 integrin regulates epithelial proliferation and cell shape during alveolar repair

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