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Transcription profiling of skin from wild type and Hoxc13 transgenic mice to investigate their propensity to develop severe hair growth defects and alopecia

ABSTRACT: A study of the effects of Hoxc13 overexpression in skin using Hoxc13 transgenic mice (GC13) known to develop severe hair growth defects and alopecia. Total skin from GC13 mice 5 dpn is compared to total skin from normal FVB mice 5 dpn using Affymetrix HG-U74Av2 GeneChips.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Awgulewitsch

PROVIDER: E-GEOD-2374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epididymal cysteine-rich secretory protein 1 encoding gene is expressed in murine hair follicles and downregulated in mice overexpressing Hoxc13.

Peterson Ron L RL Tkatchenko Tatiana V TV Pruett Nathanael D ND Potter Christopher S CS Jacobs Donna F DF Awgulewitsch Alexander A

The journal of investigative dermatology. Symposium proceedings 20051201 3

Members of the Hox gene family of transcriptional regulators are believed to play essential roles in hair follicle differentiation, although little is known about the molecular mechanisms mediating these putative control functions. Transgenic mice overexpressing Hoxc13 in hair follicles (GC13 mice) exhibit complex phenotypic alterations including hair shaft defects and alopecia, as well as severe epidermal abnormalities. To identify some of the genetic pathways affected by Hoxc13 overexpression ...[more]

PMID: 16382673

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Hoxc13 overexpression in skin

Project description:A study of the effects of Hoxc13 overexpression in skin using Hoxc13 transgenic mice (GC13) known to develop severe hair growth defects and alopecia. Total skin from GC13 mice 5 dpn is compared to total skin from normal FVB mice 5 dpn using Affymetrix HG-U74Av2 GeneChips. Keywords: repeat sample

2006-03-07 | GSE2374 | GEO

Prostaglandin D2 Inhibits Hair Growth and Is Elevated in Bald Scalp of Men with Androgenetic Alopecia

Project description:Testosterone is necessary for the development of male pattern baldness, known as androgenetic alopecia (AGA); yet the mechanisms for decreased hair growth in this disorder are unclear. Here, we show that prostaglandin D2 synthase (PTGDS) is elevated at the mRNA and protein levels in bald scalp compared to haired scalp of men with AGA. The product of PTGDS enzyme activity, prostaglandin D2 (PGD2), is similarly elevated in bald scalp. During normal follicle cycling in mice Ptgds and PGD2 levels increase immediately preceding the regression phase, suggesting an inhibitory effect on hair growth. We show that PGD2 inhibits hair growth in explanted human hair follicles and when applied topically to mice. Hair growth inhibition requires the PGD2 receptor G protein-coupled receptor 44 (GPR44), but not the prostaglandin D2 receptor 1(PTGDR). Furthermore, we find that a transgenic mouse, K14-Ptgs2, which targets prostaglandin-endoperoxide synthase 2 expression to the skin, demonstrates elevated levels of PGD2 in the skin and develops alopecia, follicular miniaturization and sebaceous gland hyperplasia, which are all hallmarks of human AGA. These results define PGD2 as an inhibitor of hair growth in AGA and suggest the PGD2-GPR44 pathway as a potential target for treatment. 5 individuals with Androgenetic Alopecia were biopsied at both their haired and bald scalp for mRNA purification and microarray (total 10 arrays)

2012-03-26 | E-GEOD-36169 | biostudies-arrayexpress

First ruxolitinib treatment of human alopecia areata patients

Project description:Two patients with alopecia areata were treated with systemic ruxolitinib. Skin biopsies were taken before starting treatment and 12 weeks after starting treatment. We used microarrays to assess changes in gene expression of affected skin before and after starting treatment Two patients with alopecia areata were recruited for our study. Skin biopsies of affected scalp were taken prior to starting treatment with oral ruxolinitib. Additional skin biopsies were taken 12 weeks after starting treatment. Scalp skin biopsies were taken from patients without alopecia areata for comparison. RNA was extracted, cDNA libraries were made and profiled on affymetrix microarray chips.

2014-08-17 | E-GEOD-58573 | biostudies-arrayexpress

Spatial transcriptomics of autoimmune alopecia

Project description:Autoimmune alopecia is a prevalent, highly morbid disease. The inflammatory pathways causing hair loss are not well characterized. We profiled two tissue microarrays comparing healthy skin to scarring alopecia and alopecia areata via spatial transcriptomics to analyze the genes and pathways dysregulated in autoimmune alopecia in direct proximity to the hair follicle.

2023-09-25 | GSE227632 | GEO

Human Alopecia Areata Skin Profiling

Project description:Our goal was to develop a transcriptomic description of affected alopecic scalp skin from patients with alopecia areata. 5 biological replicates of skin samples from 5 separate AA patients compared with 5 similar scalp samples from healthy control patients

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Prostaglandin D2 Inhibits Hair Growth and Is Elevated in Bald Scalp of Men with Androgenetic Alopecia

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Human Alopecia Areata Skin Profiling

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Transgenic overexpression of Pla2g2f at a level relevant to skin pathology display alopecia and psoriasis-like epidermal hyperplasia

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2015-08-08 | E-GEOD-71829 | biostudies-arrayexpress

Microneedle-mediated Delivery of Immunomodulators Restores Immune Privilege in Hair Follicles and Reverses Immune-Mediated Alopecia

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2024-05-13 | GSE267295 | GEO

miRNA profiling of C3H/HeJ mice with and without spontaneous alopecia areata

Project description:Our goal was to identify miRNA expression patterns that correlated with spontaneous onset of autoimmune alopecia in C3H/HeJ mice compare to normal haired controls. Skin from 3 mice with or without spontaneous AA were taken for the study.

2017-02-03 | GSE94407 | GEO

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