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Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 1)


ABSTRACT: In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles. Gene expression was measured in 7 C. glabrata laboratory strains (SFY101, SFY103, SFY105, SFY109, SFY111, SFY115, SFY116) expressing different CgPDR1 hyperactive alleles, in 1 strain (SFY114) expressing a wild-type CgPDR1 allele and in one strain deleted for CgPDR1. The one-color system was used. 3 independent experiments were performed using 3 biological replicates for each strain.

ORGANISM(S): Candida glabrata

SUBMITTER: Dominique Sanglard 

PROVIDER: E-GEOD-23827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata.

Ferrari Sélène S   Sanguinetti Maurizio M   Torelli Riccardo R   Posteraro Brunella B   Sanglard Dominique D  

PloS one 20110309 3


In Candida glabrata, the transcription factor CgPdr1 is involved in resistance to azole antifungals via upregulation of ATP binding cassette (ABC)-transporter genes including at least CgCDR1, CgCDR2 and CgSNQ2. A high diversity of GOF (gain-of-function) mutations in CgPDR1 exists for the upregulation of ABC-transporters. These mutations enhance C. glabrata virulence in animal models, thus indicating that CgPDR1 might regulate the expression of yet unidentified virulence factors. We hypothesized  ...[more]

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