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Hepatic Gene Expression Profiling of 5'AMP Induced Hypometabolism in Mice

ABSTRACT: Mice injected IP with 5'-AMP enter a hypometabolic state that is an induced hybernation (AIHM). Recovery from this AIHM is marked by reverse flip (RF) behavior Illumina arrays were used to profile control, AIHM and RF aminals in RNA prepared from the liver. Animals were injected with vehicle or 0.5 mg/g body weight 5'-adenosine monophosphate. At 6 hours (AIHM) or 12 hours (RF) animals were killed and total RNA prepared from the liver.

ORGANISM(S): Mus musculus

SUBMITTER: David Loose

PROVIDER: E-GEOD-23975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hepatic gene expression profiling of 5'-AMP-induced hypometabolism in mice.

Zhao Zhaoyang Z Miki Takao T Van Oort-Jansen Anita A Matsumoto Tomoko T Loose David S DS Lee Cheng Chi CC

Physiological genomics 20110111 7

There is currently much interest in clinical applications of therapeutic hypothermia. Hypothermia can be a consequence of hypometabolism. We have recently established a procedure for the induction of a reversible deep hypometabolic state in mice using 5'-adenosine monophosphate (5'-AMP) in conjunction with moderate ambient temperature. The current study aims at investigating the impact of this technology at the gene expression level in a major metabolic organ, the liver. Our findings reveal that ...[more]

PMID: 21224422

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