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MicroRNA Expression in Human RPE Cells in Culture: Control vs Treated with IFN-? + TNF-? + IL-1?

ABSTRACT: Human retinal pigment epithelial (HRPE) cells in culture respond to inflammatory cytokines (IFN-? + TNF-? + IL-1? ) by increasing the expression of many cytokines and chemokines. The goal of this study was to delineate the role of miRNA in this process. We employed microarray analysis to study the effect of inflammatory cytokines on the miRNA expression in HRPE cells. HRPE cells were treated with a mixture of IFN-? (100 u), TNF-? (10 ng/ml) and IL-1? (10 ng/ml) for 16 hours, and the miRNA expression was analyzed using RNA preparations isolated from control and treated cells. The experiment was repeated. Control samples were labeled with Cy3 while the treated samples with Cy5.

ORGANISM(S): Homo sapiens

SUBMITTER: R Kutty

PROVIDER: E-GEOD-23979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Inflammatory cytokines regulate microRNA-155 expression in human retinal pigment epithelial cells by activating JAK/STAT pathway.

Kutty R Krishnan RK Nagineni Chandrasekharam N CN Samuel William W Vijayasarathy Camasamudram C Hooks John J JJ Redmond T Michael TM

Biochemical and biophysical research communications 20101013 2

Inflammatory response of the retinal pigment epithelium plays a critical role in the pathogenesis of retinal degenerative diseases such as age-related macular degeneration. Our previous studies have shown that human retinal pigment epithelial (HRPE) cells, established from adult donor eyes, respond to inflammatory cytokines by enhancing the expression of a number of cytokines and chemokines. To investigate the role of microRNA (miRNA) in regulating this response, we performed microarray analysis ...[more]

PMID: 20950585

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