Genome wide analysis of Saccharomyces nascent RNA
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces
SUBMITTER: Fernando Carrillo Oesterreich
PROVIDER: E-GEOD-24040 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Global analysis of nascent RNA reveals transcriptional pausing in terminal exons.
Carrillo Oesterreich Fernando F Preibisch Stephan S Neugebauer Karla M KM
Molecular cell 20101101 4
Pre-mRNA splicing is catalyzed by the spliceosome, which can assemble on pre-mRNA cotranscriptionally. However, whether splicing generally occurs during transcription has not been addressed. Indeed, splicing catalysis is expected to occur posttranscriptionally in yeast, where the shortness of terminal exons should leave insufficient time for splicing. Here, we isolate endogenous S. cerevisiae nascent RNA and determine gene-specific splicing efficiencies and transcription profiles, using high-den ...[more]
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