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Expression data in whole Arabidopsis seedlings after treatment with the herbicide dicamba

ABSTRACT: Dicamba is an auxin-like herbicide that can stimulate the production of ethylene and ABA biosynthesis. The subsequent stomatal closure and build-up of reactive oxygen species is hypothesized to contribute to plant death. In order to further understand the herbicide dicamba's mode of action at the molecular level, we used microarrays to dissect global gene expression in Arabidopsis seedlings 10 hrs after dicamba treatment. 4 day old Arabidopsis seedlings (a Columbia line containing a -800GSTF8::LUC reporter) were flooded with water or 7mM dicamba for 40 minutes before the treatment was poured off. Ten hours after the start of the treatment, whole seedlings were collected

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Cynthia Gleason

PROVIDER: E-GEOD-24052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mutant analysis in Arabidopsis provides insight into the molecular mode of action of the auxinic herbicide dicamba.

Gleason Cynthia C Foley Rhonda C RC Singh Karam B KB

PloS one 20110308 3

Herbicides that mimic the natural auxin indole-3-acetic acid are widely used in weed control. One common auxin-like herbicide is dicamba, but despite its wide use, plant gene responses to dicamba have never been extensively studied. To further understand dicamba's mode of action, we utilized Arabidopsis auxin-insensitive mutants and compared their sensitivity to dicamba and the widely-studied auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The mutant axr4-2, which has disrupted auxin t ...[more]

PMID: 21408147

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