Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hypoxia, ketoconazole, and the UPC2 gene in Candida albicans

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE24073: Transcriptional profile of Candida albicans during Hypoxic conditions. GSE24074: Transcriptional profile of Candida albicans DAY286 culture without ketoconazole versus DAY286 culture with 0.04 Î¼g/ml ketoconazole, both at 20% oxygen (normoxia). GSE24075: Transcriptional profile of Candida albicans DAY286 versus UPC2 delete, both at 1% oxygen (hypoxia). Refer to individual Series

ORGANISM(S): Candida albicans

SUBMITTER: Alessandro Guida

PROVIDER: E-GEOD-24076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Regulation of the hypoxic response in Candida albicans.

Synnott John M JM Guida Alessandro A Mulhern-Haughey Siobhan S Higgins Desmond G DG Butler Geraldine G

Eukaryotic cell 20100924 11

The regulation of the response of Candida albicans to hypoxic (low-oxygen) conditions is poorly understood. We used microarray and other transcriptional analyses to investigate the role of the Upc2 and Bcr1 transcription factors in controlling expression of genes involved in cell wall metabolism, ergosterol synthesis, and glycolysis during adaptation to hypoxia. Hypoxic induction of the ergosterol pathway is mimicked by treatment with sterol-lowering drugs (ketoconazole) and requires UPC2. Expre ...[more]

PMID: 20870877

Similar Datasets

Transcriptional profile of Candida albicans during Hypoxic conditions.

Project description:Whole genome microarray were used to generate the transcriptional profile of C.albicans in hypoxic condition. RNA was isolated from DAY286 grown in YPD medium in normoxia or hypoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Eight independent biological replicates were compared. 2 dye swaps were perfomed so that 6 out of 8 samples isolated from normoxic culture were labeled with Cy3, and 2 were labeled with Cy5.

2010-11-15 | E-GEOD-24073 | biostudies-arrayexpress

Transcriptional profile of Candida albicans DAY286 culture without ketoconazole versus DAY286 culture with 0.04 Î¼g/ml ketoconazole, both at 20% oxygen (normoxia).

Project description:Whole genome microarray were used to generate the transcriptional profile of C.albicans in the presence of ketoconazole. RNA was isolated from DAY286 grown with or without ketoconazole in YPD medium in normoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. 2 dye swaps were perfomed so that 2 out of 4 samples isolated from DAY286 in normoxic culture without ketoconazole were labeled with Cy3, and 2 were labeled with Cy5.

2010-11-15 | E-GEOD-24074 | biostudies-arrayexpress

Transcriptional profile of Candida albicans DAY286 versus UPC2 delete, both at 1% oxygen (hypoxia).

Project description:Whole genome microarray were used to generate the transcriptional profile of C.albicans UPC2 delete in hypoxia. RNA was isolated from DAY286 or UPC2 delete grown in YPD medium in hypoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Nine independent biological replicates were compared. 3 dye swaps were perfomed so that 6 out of 9 samples isolated from DAY286 in hypoxic culture were labeled with Cy3, and 3 were labeled with Cy5.

2010-11-15 | E-GEOD-24075 | biostudies-arrayexpress

Transcriptional profile of Candida parapsilosis CLIB214 versus UPC2 delete, both at 1% oxygen (hypoxia)

Project description:Whole genome microarray were used to generate the transcriptional profile of C. parapsilosis UPC2 delete in hypoxia. RNA was isolated from CLIB214 or upc2 delete grown in YPD medium in hypoxia at 30 degree Celsius for 2 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. 2 dye swaps were perfomed so that 2 out of 4 samples isolated from CLIB214 in hypoxic culture were labeled with Cy3, and 2 were labeled with Cy5

2011-11-30 | E-GEOD-32713 | biostudies-arrayexpress

Transcriptional profile of Candida parapsilosis CLIB214 21% Oxygen (normoxia) versus at 1% oxygen (hypoxia).

Project description:Whole genome microarray were used to generate the transcriptional profile of C. parapsilosis in hypoxic condition. RNA was isolated from CLIB214 grown in YPD medium in normoxia or hypoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. Isolates from normoxic condition were labeled with Cy3 and isolates from hypoxic condition were labeled with Cy5.

2011-11-30 | E-GEOD-32712 | biostudies-arrayexpress

Transcriptional landscape of the pathogenic yeast Candida parapsilosis

Project description:This SuperSeries is composed of the following subset Series: GSE32712: Transcriptional profile of Candida parapsilosis CLIB214 21% Oxygen (normoxia) versus at 1% oxygen (hypoxia). GSE32713: Transcriptional profile of Candida parapsilosis CLIB214 versus UPC2 delete, both at 1% oxygen (hypoxia) GSE32714: Transcriptional landscape of Candida parapsilosis Refer to individual Series

2011-11-30 | E-GEOD-32716 | biostudies-arrayexpress

Transcriptional profile of Candida albicans bcr1 knockout.

Project description:Whole genome microarrays were used to compare the transcriptional profile of Candida albicans bcr1 knockout to wild type cells. RNA was isolated from DAY286 (wild type) or bcr1 delete (CJN702) grown in SD medium supplemented with 50 mM glucose and 10% feotal calf serum and labeled with Cy3 or Cy5. Four independent biological replicates were compared. Two dye swaps were perfomed so that two of the four DAY286 cultures were labeled with Cy3, and two were labeled with Cy5.

2011-11-04 | E-GEOD-33460 | biostudies-arrayexpress

Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans

Project description:This SuperSeries is composed of the following subset Series: GSE33460: Transcriptional profile of Candida albicans bcr1 knockout. GSE33461: Transcriptional profile of Candida parapsilosis bcr1 knockout. GSE33462: Transcriptional profile of Candida parapsilosis CLIB214 culture in low iron conditions Refer to individual Series

2011-11-05 | E-GEOD-33490 | biostudies-arrayexpress

Transcription profiling of human umbilical vein endothelial cells responses to Candida albicans hyphae and blastospores

Project description:We perform microarray analysis of HUVECs upon stimulation with virulent wildtype C. albicans strain SC5314 or its efg1/efg1 cph1/cph1 hyphal-deficient derivative strain CAN34 to compare the gene expression profiles elicited from HUVECs in response to these strains. In addition, these responses are compared to that of TNF-alpha induced responses to determine which responses are Candida-specific. Experiment Overall Design: HUVECs are co-cultured for 3 or 8 hours in M199 medium alone or with CAN34, SC5314, or TNF-alpha. Total RNA is isolated, cRNA is synthesized and labeled, and labeled cRNA is hybridized onto Affymetrix chips. Each Candida and TNF sample is compared to its corresponding medium alone sample to determine fold changes in expression at each time point.

2008-06-16 | E-GEOD-8166 | biostudies-arrayexpress

MASH Explorer: A Universal Software Environment for Top-Down Proteomics

Project description:MASH Explorer, a universal, comprehensive, and user-friendly software environment for top-down proteomics. MASH Explorer integrates multiple spectral deconvolution and database searching algorithms into a single, universal platform which can process top-down proteomics data from various vendor formats, for the first time. It addresses the urgent need in the rapidly growing top-down proteomics community and is freely available to all users worldwide. With the critical need and tremendous support from the community, we envision this MASH Explorer software package will play an integral role in advancing top-down proteomics to realize its full potential for biomedical research.

2021-09-09 | PXD020615 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data