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Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila

ABSTRACT: Combining RNAi in cultured cells and analysis of mutant animals, we probed roles of known piRNA pathway components in the initiation and effector phases of transposon silencing. total RNA and RNA associated with Piwi was isolated and size-fractionated by PAGE into 19-29nt. These were processed and sequenced on Illumina Genome Analyzer II.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Astrid Haase

PROVIDER: E-GEOD-24108 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila.

Haase Astrid D AD Fenoglio Silvia S Muerdter Felix F Guzzardo Paloma M PM Czech Benjamin B Pappin Darryl J DJ Chen Caifu C Gordon Assaf A Hannon Gregory J GJ

Genes & development 20101021 22

Combining RNAi in cultured cells and analysis of mutant animals, we probed the roles of known Piwi-interacting RNA (piRNA) pathway components in the initiation and effector phases of transposon silencing. Squash associated physically with Piwi, and reductions in its expression led to modest transposon derepression without effects on piRNAs, consistent with an effector role. Alterations in Zucchini or Armitage reduced both Piwi protein and piRNAs, indicating functions in the formation of a stable ...[more]

PMID: 20966049

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Production of artificial piRNAs in flies and mice

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FLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)

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