Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of murine lungs to investigate the role of mechanical ventilation in modulating lungs transcriptional response to lipopolysaccharide (LPS)

ABSTRACT: This experiment investigated the role of mechanical ventilation (MV) in modulating lung's transcriptional response to LPS. Twenty four C57/B6 male mice were randomized to four groups: 1. Control, 2. MV, 3. LPS and 4. MV+LPS. Expression profiling of whole lungs revealed a significant augmentation of the transcriptional response in the combined MV+LPS group relative to the other 3 conditions.

ORGANISM(S): Mus musculus

SUBMITTER: Sina Gharib

PROVIDER: E-GEOD-2411 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Modulation of lipopolysaccharide-induced gene transcription and promotion of lung injury by mechanical ventilation.

Altemeier William A WA Matute-Bello Gustavo G Gharib Sina A SA Glenny Robb W RW Martin Thomas R TR Liles W Conrad WC

Journal of immunology (Baltimore, Md. : 1950) 20050901 5

Mechanical ventilation (MV) with tidal volumes of 10-12 ml/kg is considered safe in the absence of acute lung injury (ALI). However, recent studies show that, when lung injury is already present, tidal volumes of this magnitude increase inflammation and injury in the lungs. We hypothesized that MV with tidal volumes of 10-ml/kg can also function as a cofactor in the initiation of ALI by modulating the transcriptional response to bacterial products. To test this hypothesis, we developed a mouse m ...[more]

PMID: 16116230

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Dataset Information

Transcription profiling of murine lungs to investigate the role of mechanical ventilation in modulating lungs transcriptional response to lipopolysaccharide (LPS)

Publications

Modulation of lipopolysaccharide-induced gene transcription and promotion of lung injury by mechanical ventilation.

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