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ChIP-Seq of chromatin marks at distal enhancers in Mouse Embryonic Stem Cells and adult tissues.

ABSTRACT: Enhancers are enriched by histone H3 lysine 4 mono methylation (H3K4me1). To gain insight into the relation between enhancers and developmental state, chromatin immunoprecipitation coupled with massive parallel sequencing (ChIP-seq) was performed in several cell types to determine the genome-wide binding targets of H3K4me1 and several other possible enhancer associated modifications. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A chromatin IP against histone H3 or whole cell extract was sequenced and used as the background to determine enrichment. ChIP was performed mainly using antibodies against the chromatin marks H3K4me1 and H3K27ac.

ORGANISM(S): Mus musculus

SUBMITTER: Menno Creyghton

PROVIDER: E-GEOD-24164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone H3K27ac separates active from poised enhancers and predicts developmental state.

Creyghton Menno P MP Cheng Albert W AW Welstead G Grant GG Kooistra Tristan T Carey Bryce W BW Steine Eveline J EJ Hanna Jacob J Lodato Michael A MA Frampton Garrett M GM Sharp Phillip A PA Boyer Laurie A LA Young Richard A RA Jaenisch Rudolf R

Proceedings of the National Academy of Sciences of the United States of America 20101124 50

Developmental programs are controlled by transcription factors and chromatin regulators, which maintain specific gene expression programs through epigenetic modification of the genome. These regulatory events at enhancers contribute to the specific gene expression programs that determine cell state and the potential for differentiation into new cell types. Although enhancer elements are known to be associated with certain histone modifications and transcription factors, the relationship of these ...[more]

PMID: 21106759

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