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The transcriptional modulator H2AFY marks Huntington's disease activity in men and mice

ABSTRACT: To accelerate the development of disease-modifying therapeutics for Huntington’s disease (HD), a dynamic biomarker of disease activity and treatment response is critically needed. Venous cellular whole blood from 8 patients with Huntington's disease (HD) and 6 control subjects were collected in PAXgene tubes (Wiagen, Valencia, CA). RNA was isolated from whole blood using the PAXgene blood RNA kit (Qiagene) with DNase treatment. The biotinylated complementary RNA was fragmented and hybridized to Affymetrix human genome U133A microarrays. The Affymetrix .CEL files were normalized to “all probe sets” in a standardized matter, and scaled to 100 by the MAS5 5.0 algorithm implemented in the Bioconductor package.

ORGANISM(S): Homo sapiens

SUBMITTER: Bin Zheng

PROVIDER: E-GEOD-24250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional modulator H2A histone family, member Y (H2AFY) marks Huntington disease activity in man and mouse.

Hu Yi Y Chopra Vanita V Chopra Raman R Locascio Joseph J JJ Liao Zhixiang Z Ding Hongliu H Zheng Bin B Matson Wayne R WR Ferrante Robert J RJ Rosas H Diana HD Hersch Steven M SM Scherzer Clemens R CR

Proceedings of the National Academy of Sciences of the United States of America 20111003 41

Huntington disease (HD) is a progressive neurodegenerative disease that affects 30,000 individuals in North America. Treatments that slow its relentless course are not yet available, and biomarkers that can reliably measure disease activity and therapeutic response are urgently needed to facilitate their development. Here, we interrogated 119 human blood samples for transcripts associated with HD. We found that the dynamic regulator of chromatin plasticity H2A histone family, member Y (H2AFY) is ...[more]

PMID: 21969577

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The transcriptional modulator H2AFY marks Huntington's disease activity in men and mice

Project description:To accelerate the development of disease-modifying therapeutics for Huntington’s disease (HD), a dynamic biomarker of disease activity and treatment response is critically needed.

2011-09-01 | GSE24250 | GEO

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