Unknown,Transcriptomics,Genomics,Proteomics

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Sprouting transcriptome in cortical neurons: young


ABSTRACT: Selective isolation of total RNA and then whole genome expression analysis of sprouting neurons in peri-infarct cortex of the adult rat after stroke, compared to adjacent neurons that have not sprouted a new connection, in young adult (2 months) and aged (2 years) animals. Two different fluorescent conjugates of the tracer cholera toxin B (CTb), CTb-Alexa 488 and CTb-Alexa 647 (Molecular Probes), were sequentially injected into peri-infarct cortex at the sites of post-stroke axonal sprouting using a picospritzer pressure injection system. After survival periods of 7 days and 21 days (separate cohorts of animals), CTb-647 was injected exactly within CTb-488 injection site. Seven days after the second tracer injection, laser capture microdissection (LCM) was used to capture ~300 neurons for each cell type. Total RNA was isolated from captured cells and subjected to two rounds of T7 amplification.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Giovanni Coppola 

PROVIDER: E-GEOD-24440 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An age-related sprouting transcriptome provides molecular control of axonal sprouting after stroke.

Li Songlin S   Overman Justine J JJ   Katsman Diana D   Kozlov Serguei V SV   Donnelly Christopher J CJ   Twiss Jeffery L JL   Giger Roman J RJ   Coppola Giovanni G   Geschwind Daniel H DH   Carmichael S Thomas ST  

Nature neuroscience 20101107 12


Stroke is an age-related disease. Recovery after stroke is associated with axonal sprouting in cortex adjacent to the infarct. The molecular program that induces a mature cortical neuron to sprout a new connection after stroke is not known. We selectively isolated neurons that sprout a new connection in cortex after stroke and compared their whole-genome expression profile to that of adjacent, non-sprouting neurons. This 'sprouting transcriptome' identified a neuronal growth program that consist  ...[more]

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