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Transcription profiling of Arabidopsis inflorescence stages 1-12 from small RNA biogenesis mutants

ABSTRACT: Inflorescence stages 1 to 12 from mutants involved in Arabidopsis small RNA metabolism. Three biological replicates of each mutant comprising at least 9 independent plants were harvested, and the expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the sample groups allow the identification of genes regulated by small RNAs (microRNAs and siRNAs).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Edwards Allen

PROVIDER: E-GEOD-2473 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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microRNA-directed phasing during trans-acting siRNA biogenesis in plants.

Allen Edwards E Xie Zhixin Z Gustafson Adam M AM Carrington James C JC

Cell 20050401 2

Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation. In plants, miRNAs and trans-acting (ta) siRNAs form through distinct biogenesis pathways, although they both interact with target transcripts and guide cleavage. An integrated approach to identify targets of Arabidopsis thaliana miRNAs and ta-siRNAs revealed several new classes of small RNA-regulated genes, including conventional genes such as Argonaute2 and an E2-ubiqui ...[more]

PMID: 15851028

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Small RNA biogenesis mutants

Project description:Inflorescence stages 1 to 12 from mutants involved in Arabidopsis small RNA metabolism. Three biological replicates of each mutant comprising at least 9 independent plants were harvested, and the expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the sample groups allow the identification of genes regulated by small RNAs (microRNAs and siRNAs). Keywords: other

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