Peripheral blood gene expression changes during allergen inhalation challenge in atopic asthmatic individuals

ABSTRACT: Background: Allergen inhalation challenge in mild asthmatics induces airflow obstruction, airway hyperresponsiveness and inflammation, providing a model for hypothesis-generating experiments to understand regulation of these responses. We have sought to evaluate the peripheral whole blood transcriptome, post-challenge compared to pre-challenge, and to determine the effect of globin mRNA reduction methodology. Methods: Asthmatic subjects (20-60 years of age, with stable, mild allergic asthma, n=17) underwent allergen inhalation challenges. All subjects had an early asthmatic response of ≥ 20% fall in FEV1; most had a late phase response of ≥ 15% fall in FEV1. Blood was collected immediately prior to, and two hours after allergen challenge. Transcriptome analysis was performed using Af

ORGANISM(S): Homo sapiens

SUBMITTER: Scott Tebbutt 

PROVIDER: E-GEOD-24745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress