Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient DCs)


ABSTRACT: Effective anti-viral immunity depends on the ability of infected cells or cells triggered with virus-derived nucleic acids to produce type I interferon (IFN), which activates transcription of numerous antiviral genes. However, disproportionately strong or chronic IFN expression is a common cause of inflammatory and autoimmune diseases. Here we describe an epigenetic mechanism that determines cell-type specific differences in IFN and IFN-stimulated gene expression in response to exogenous signals. We identify di-methylation of histone H3 at lysine 9 (H3K9me2) as a suppressor of IFN and IFN-inducible antiviral gene expression. We show that levels of H3K9me2 at IFN and IFN stimulated genes (ISG) correlate inversely with the scope and amplitude of IFN and ISG expression in fibroblasts and dendritic cells. Accordingly, genetic ablation or pharmacological inactivation of lysine methyltransferase G9a, which is essential for the generation of H3K9me2, resulted in phenotypic conversion of fibroblasts into highly potent IFN-producing cells and rendered these cells resistant to pathogenic RNA viruses. In summary, our studies implicate H3K9me2 and enzymes controlling its abundance as key regulators of innate antiviral immunity. Examination of gene expression in response to PolyI:C in WT and G9a deficient DCs

ORGANISM(S): Mus musculus

SUBMITTER: Terry Fang 

PROVIDER: E-GEOD-24774 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-03-02 | E-GEOD-22102 | biostudies-arrayexpress
2012-03-02 | E-GEOD-24776 | biostudies-arrayexpress
2012-03-02 | E-GEOD-24826 | biostudies-arrayexpress
2012-03-02 | GSE24776 | GEO
2012-03-02 | GSE24774 | GEO
2012-03-02 | GSE22102 | GEO
2013-06-01 | E-GEOD-45698 | biostudies-arrayexpress
2013-06-01 | E-GEOD-45679 | biostudies-arrayexpress
2013-12-14 | E-GEOD-53312 | biostudies-arrayexpress
2013-06-01 | E-GEOD-45681 | biostudies-arrayexpress