Unknown,Transcriptomics,Genomics,Proteomics

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Small non-coding RNA expression in murine germ cells


ABSTRACT: In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in a bovine serum albumin gradient. The small RNA fraction (18-36nt) was cloned and sequenced from total RNA of each cell type. Three types of mouse germ cells small RNAs were used for high throughput sequencing analysis

ORGANISM(S): Mus musculus

SUBMITTER: Chunsheng han 

PROVIDER: E-GEOD-24822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

piRNA profiling during specific stages of mouse spermatogenesis.

Gan Haiyun H   Lin Xiwen X   Zhang Zhuqiang Z   Zhang Wei W   Liao Shangying S   Wang Lixian L   Han Chunsheng C  

RNA (New York, N.Y.) 20110520 7


PIWI-interacting RNAs (piRNAs) are a class of small RNAs abundantly expressed in animal gonads. piRNAs that map to retrotransposons are generated by a "ping-pong" amplification loop to suppress the activity of retrotransposons. However, the biogenesis and function of other categories of piRNAs have yet to be investigated. In this study, we first profiled the expression of small RNAs in type A spermatogonia, pachytene spermatocytes, and round spermatids by deep sequencing. We then focused on the  ...[more]

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