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Transcriptional responses of Acinetobacter baumannii ATCC 17978 to limiting iron availability

ABSTRACT: A. baumannii ATCC 17978 cells were incubated under iron replete (mueller-hinton) and iron limiting (MH + 200 ÂµM 2,2'-dipyridyl) conditions, total RNA was extracted when cultures reached OD600=0.7. The probes on the microarray cover all predicted open reading frames (at least 4 per ORF) and additional replicates of housekeeping genes of the A. baumannii ATCC 17978 genome

ORGANISM(S): Acinetobacter baumannii

SUBMITTER: Bart Eijkelkamp

PROVIDER: E-GEOD-24921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions.

Eijkelkamp Bart A BA Hassan Karl A KA Paulsen Ian T IT Brown Melissa H MH

BMC genomics 20110223

<h4>Background</h4>Iron acquisition systems are important virulence factors in pathogenic bacteria. To identify these systems in Acinetobacter baumannii, the transcriptomic response of the completely sequenced strain ATCC 17978 under iron limiting conditions was investigated using a genomic microarray that contained probes for all annotated open reading frames.<h4>Results</h4>Under low iron conditions, transcription levels were more than 2-fold up-regulated for 463 genes, including 95 genes that ...[more]

PMID: 21342532

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