Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription in adult mouse tissues

ABSTRACT: We used Affymetrix Gene Arrays (1.0 ST) to compare gene expression across different murine tissues. Arrays were used to find disallowed genes in tissues. Different tissues were dissected from 10-12 week old C57Bl6 mice for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Lieven Thorrez

PROVIDER: E-GEOD-24940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Tissue-specific disallowance of housekeeping genes: the other face of cell differentiation.

Thorrez Lieven L Laudadio Ilaria I Van Deun Katrijn K Quintens Roel R Hendrickx Nico N Granvik Mikaela M Lemaire Katleen K Schraenen Anica A Van Lommel Leentje L Lehnert Stefan S Aguayo-Mazzucato Cristina C Cheng-Xue Rui R Gilon Patrick P Van Mechelen Iven I Bonner-Weir Susan S Lemaigre Frédéric F Schuit Frans F

Genome research 20101118 1

We report on a hitherto poorly characterized class of genes that are expressed in all tissues, except in one. Often, these genes have been classified as housekeeping genes, based on their nearly ubiquitous expression. However, the specific repression in one tissue defines a special class of "disallowed genes." In this paper, we used the intersection-union test to screen for such genes in a multi-tissue panel of genome-wide mRNA expression data. We propose that disallowed genes need to be repress ...[more]

PMID: 21088282

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Project description:ABSTRACT:Pregnancy requires a higher functional beta cell mass and this is associated with profound changes in the gene expression profile of pancreatic islets. Taking Tph1 as a sensitive marker for pregnancy-related islet mRNA expression in female mice, we previously identified prolactin receptors and placental lactogen as key signalling molecules. Since beta cells from male mice also express prolactin receptors, the question arose whether male and female islets have the same phenotypic resilience at the mRNA level during pregnancy. We addressed this question in vitro, by using islet tissue culture with placental lactogen and in vivo, by transplanting male or female islets into female acceptor mice. Additionally, the islet mRNA expression of pregnant prolactin receptor deficient mice was compared with that of their pregnant wild-type littermates. When cultured with placental lactogen, or transplanted in female recipients that became pregnant (day 12.5), male islets induced the ‘islet pregnancy gene signature’, which we defined as the 12 highest induced genes in non-transplanted female islets at day 12.5 of pregnancy. In addition, serotonin immunoreactivity was also induced in these male transplanted islets at day 12.5 of pregnancy. In order to investigate the importance of prolactin receptors in these mRNA changes we used a prolactin receptor deficient mouse model. For the 12 genes of the signature, which are highly induced in control pregnant mice, no significant induction of mRNA transcripts was found at day 9.5 of pregnancy. Together, our results support the key role of placental lactogen as a circulating factor that can trigger the pregnancy mRNA profile in male and female beta cells. The data obtained from the normal islets of pregnant mice (day12.5) was already described in Schraenen et al. 2010 (PMID: 20886204 and PMID: 20938637). Islets and islet grafts were isolated from non-prengant and pregnant mice for RNA extraction and hybridization on Affymetrix microarrays. For every condition, at least 3 biological replicates were used.

Dataset Information

Transcription in adult mouse tissues

Publications

Tissue-specific disallowance of housekeeping genes: the other face of cell differentiation.

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