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DRNA-Seq of Chlamydia pneumoniae

ABSTRACT: The obligate intracellular human pathogen Chlamydia pneumoniae was subjected to dRNA-Seq to gain insights into the transcriptome. The two distinct life cycle forms elementary bodies (EB) and reticulate bodies (RB) were isolated from human Hep2 cell line by differential gradient centrifugation. Total RNA was isolated and partially treated with Terminator Exonuclease to digest RNA without 5'-PPP and thereby enrich for native 5' ends.

ORGANISM(S): Chlamydophila pneumoniae CWL029

SUBMITTER: Marco Albrecht

PROVIDER: E-GEOD-24999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcriptional landscape of Chlamydia pneumoniae.

Albrecht Marco M Sharma Cynthia M CM Dittrich Marcus T MT Müller Tobias T Reinhardt Richard R Vogel Jörg J Rudel Thomas T

Genome biology 20111011 10

<h4>Background</h4>Gene function analysis of the obligate intracellular bacterium Chlamydia pneumoniae is hampered by the facts that this organism is inaccessible to genetic manipulations and not cultivable outside the host. The genomes of several strains have been sequenced; however, very little information is available on the gene structure and transcriptome of C. pneumoniae.<h4>Results</h4>Using a differential RNA-sequencing approach with specific enrichment of primary transcripts, we defined ...[more]

PMID: 21989159

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dRNA-Seq of Chlamydia pneumoniae

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Supermajority of Chlamydia trachomatis genes are activated during the first hour of infection

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Project description:dRNA-Seq of Chlamydia pneumoniae

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