Study for evaluating the effect of cold ischemic time and RNA stabilization method on RNA integrity and gene expression measurements
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Christos Hatzis
PROVIDER: E-GEOD-25011 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers.
Hatzis Christos C Sun Hongxia H Yao Hui H Hubbard Rebekah E RE Meric-Bernstam Funda F Babiera Gildy V GV Wu Yun Y Pusztai Lajos L Symmans W Fraser WF
Journal of the National Cancer Institute 20111027 24
<h4>Background</h4>Reliable stability and yield of RNA from breast cancer tissues are important for biobanking, clinical trials, and diagnostic testing.<h4>Methods</h4>Aliquots of fresh primary tumor tissue from 17 surgically resected invasive breast cancers were placed into RNAlater at room temperature after tumor removal (baseline) and up to 3 hours thereafter or were snap frozen at baseline and 40 minutes thereafter. Samples were stored at -80°C until gene expression profiling with Affymetrix ...[more]
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