Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment


ABSTRACT: In this study, a newly developed 8x15K Fusarium graminearum Agilent microarray was applied to analyze transcriptional responses to azole fungicide treatment. Comparative statistical analysis of expression profiles from treated versus untreated fungal liquid cultures uncovered 1058 genes that were significantly differentially expressed. Among the 596 genes with significantly increased transcript levels, analyses using GeneOntology and FunCat annotations detected the ergosterol-biosynthesis pathway genes as the most significantly responding category, providing statistical confirmation of the mode-of-action of azole fungicides. Transcriptional profiling of 54 genes, which encode ABC transporters in F. graminearum, suggested that several of these might be involved in reducing intracellular fungicide concentration. In addition, several genes encoding transcription factors with similarity to yeast genes known to co-ordinate fungicide responses exhibited significant differential expression. Hybridization was performed with cRNA samples either derived from three independent untreated or three independent azole-treated liquid cultures (12 h at 5 mg/l tebuconazole).

ORGANISM(S): Gibberella zeae

SUBMITTER: Rayko Becher 

PROVIDER: E-GEOD-25114 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses.

Becher Rayko R   Weihmann Fabian F   Deising Holger B HB   Wirsel Stefan Gr SG  

BMC genomics 20110121


<h4>Background</h4>The toxigenic fungal plant pathogen Fusarium graminearum compromises wheat production worldwide. Azole fungicides play a prominent role in controlling this pathogen. Sequencing of its genome stimulated the development of high-throughput technologies to study mechanisms of coping with fungicide stress and adaptation to fungicides at a previously unprecedented precision. DNA-microarrays have been used to analyze genome-wide gene expression patterns and uncovered complex transcri  ...[more]

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