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Expression Profiling of Formalin-Fixed Parafin-Embedded Primary Breast Tumors DASL Cancer Panel

ABSTRACT: HER2-positive and HER2-negative samples are compared within this DASL Cancer Panel Platform Two-class comparison

ORGANISM(S): Homo sapiens

SUBMITTER: Keith Anderson

PROVIDER: E-GEOD-25231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene panels on the DASL® platform.

Reinholz Monica M MM Eckel-Passow Jeanette E JE Anderson S Keith SK Asmann Yan W YW Zschunke Michael A MA Oberg Ann L AL McCullough Ann E AE Dueck Amylou C AC Chen Beiyun B April Craig S CS Wickham-Garcia Eliza E Jenkins Robert B RB Cunningham Julie M JM Jen Jin J Perez Edith A EA Fan Jian-Bing JB Lingle Wilma L WL

BMC medical genomics 20101220

<h4>Background</h4>The cDNA-mediated Annealing, extension, Selection and Ligation (DASL) assay has become a suitable gene expression profiling system for degraded RNA from paraffin-embedded tissue. We examined assay characteristics and the performance of the DASL 502-gene Cancer Panel v1 (1.5K) and 24,526-gene panel (24K) platforms at differentiating nine human epidermal growth factor receptor 2- positive (HER2+) and 11 HER2-negative (HER2-) paraffin-embedded breast tumors.<h4>Methods</h4>Bland- ...[more]

PMID: 21172013

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